In a current article printed within the journal Scientific Studies, researchers current a novel nanobiocomposite (NBC) manufactured from agar, tragacanth gum, silk fibroin, and steel–natural framework-5 (MOF-5) for potential biomedical functions. The hydrogel was investigated for its organic exercise, together with antimicrobial properties and potential for most cancers remedy and wound therapeutic.
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Background
Hydrogels are versatile cross-linked community matrices of polymers that supply distinctive physicochemical properties, akin to hydrophilicity, tremendous absorbency, and softness. These properties make them useful in varied fields, together with drug supply, tissue engineering, and wound dressing. Pure hydrogels, derived from polymers like chitosan and alginate, stand out for his or her low cytotoxicity and biocompatibility, making them perfect for biomedical functions.
The mix of pure polymers like agar (Ag) and tragacanth gum (TG) facilitates the formation of multi-polymeric hydrogels, leveraging their ample hydroxyl purposeful teams for cross-linking. Silk fibroin (SF), derived from the Bombyx mori silkworm, is a pure protein famend for its biocompatibility, antibacterial properties, and mechanical energy, making it a promising materials in biomedicine.
The Present Examine
The chemical synthesis course of started with the preparation of Ag-TG hydrogel, the place Ag and TG had been dissolved in distilled water and combined below specified circumstances. SF was extracted from silkworm cocoons by way of a sequence of steps, together with boiling, washing, and dissolution in LiBr answer, adopted by dialysis. The Ag-TG hydrogel was then mixed with extracted SF and stirred. MOF-5 was synthesized individually and activated.
The ultimate NBC, Ag-TG hydrogel on SF mixed with MOF-5, was ready by dispersing the elements in water. The ensuing answer underwent freeze-drying and dehydration to yield the NBC for additional evaluation.
The next organic assays had been performed to evaluate the NBC’s properties:
- The Trypan blue exclusion take a look at evaluated its biocompatibility and anticancer exercise utilizing HEK293T, MCF-7, and Okay-562 cell traces.
- A hemolysis assay was used to measure blood compatibility by assessing human purple blood cell lysis proportion.
- An antibacterial assay decided the inhibition fee of E. coli and S. aureus micro organism cultures.
- Statistical evaluation utilizing SPSS Statistics 22.0 offered insights into the importance of the outcomes.
Characterization was carried out utilizing varied strategies. Organic assays had been performed utilizing an ELISA reader to guage the biocompatibility, anticancer exercise, blood compatibility, and antibacterial properties of the NBC.
Outcomes and Dialogue
The outcomes of the synthesis and characterization of the NBC are detailed under:
Fourier-transform infrared (FT-IR) evaluation: FT-IR evaluation was performed to look at the chemical bonds and purposeful teams of the synthesized NBC elements at every stage of preparation. Spectra revealed attribute indicators equivalent to the Ag-TG hydrogel, Ag-TG hydrogel/SF, and Ag-TG hydrogel/SF/MOF-5, indicating profitable synthesis and incorporation of elements.
Area-emission scanning electron microscopy (FE-SEM) imaging: FE-SEM imaging was employed to research the morphology of the NBC. Microscopic pictures confirmed the porous construction of Ag-TG hydrogel, the addition of SF biopolymer strands, and the presence of MOF-5 inside the composite, confirming the profitable synthesis and uniform distribution of elements.
Power-dispersive X-Ray (EDX) evaluation: EDX offered insights into the fundamental composition of the synthesized NBC. The spectra exhibited peaks equivalent to carbon (C), oxygen (O), nitrogen (N), and zinc (Zn), confirming the presence of Ag, TG, SF, and MOF-5 elements, respectively.
X-Ray diffraction evaluation (XRD): XRD evaluation was performed to look at the crystallinity of MOF-5 and the NBC. Patterns revealed attribute peaks equivalent to MOF-5 and a mix of crystalline MOF-5 and amorphous polymer elements within the NBC.
Thermogravimetric evaluation (TGA): TGA was carried out to guage the thermal stability and decomposition habits of the NBC. Thermograms confirmed distinct mass loss levels, adopted by the degradation of TG, Ag, and SF particular person elements.
Cell traces proliferation assay: Organic assays had been performed to evaluate the NBC’s biocompatibility and anti-cancer properties utilizing HEK293T, MCF-7, and Okay-562 cell traces. Outcomes demonstrated important inhibition of MCF-7 and Okay-562 most cancers cell proliferation, whereas HEK293T cell proliferation remained unaffected, indicating the NBC’s potential as an anti-cancer agent.
Blood compatibility: Hemolysis assay outcomes confirmed a big lytic impact of the NBC extract on purple blood cells (RBCs), indicating potential blood compatibility considerations.
Micro organism progress inhibition: The NBC exhibited important antibacterial exercise towards E. coli and S. aureus strains (with inhibition charges of 76.08 % and 69.19 %, respectively), suggesting its potential software as an antibacterial agent.
Conclusion
These findings spotlight the potential of Ag-TG hydrogel/SF/MOF-5 NBC for wound therapeutic and most cancers remedy functions. The biocompatible and non-toxic nature of the NBC, together with its enhanced antibacterial properties, make it a promising materials for future biomedical research.
Total, the research advances our understanding of nanomaterials’ potential functions in biomedical and associated fields. Additional analysis is beneficial to discover the total potential of this NBC in varied biomedical functions.
Journal Reference
Lalebeigi, F., et al. (2024). Agar-tragacanth/silk fibroin hydrogel containing Zn-based MOF as a novel nanobiocomposite with organic exercise. Scientific Studies. doi.org/10.1038/s41598-024-61329-3